Effect of the pregnant mice with Graves’ disease on thyroid function in the offspring

Methods 1. To amplify the Adenovirus Expressing TSHR A-subunit (Ad-TSHR-289) and the control adenovirus (Ad-b-gal); 2. Female Balb/c mice aged 6 weeks were divided randomly into the experimental group and the control group, then injected intramuscularly with Ad-TSHR-289 (3.3×10PFU/50μlPBS) and the control adenovirus(Adb-gal 3.3×10 PFU /50μlPBS) three times at 3 weeks intervals. 3. In 1 wk after the second injection, the levels of T4 were measured. All mice were sacrificed 4 wk after the last immunization, and then Thyroxine(T4), as well as TSH-receptor antibodies(TRAb) and the morphology of thyroid, were then examined. 4. When the immunization was over, both of groups were mated with the male mice. The experimental group were divided into three groups again. They were sacrificed at 1 d, 1 wk or 3 wk after delivery. At the same time, the breast milk from the infants’ stomach were collected. T4, as well as TRAb, Thyroid peroxidase antibodies(TPOAb), Thyroglobulin antibodies(TGAb) in serum and the morphology of thyroid, were be examined. 5. T4 were tested by radio immunoassay. TRAb, TPOAb, TGAb were tested by electrochemiluminescent immunoassay. 6. Their thyroids were removed for histological examination.